Ultrastructural view of the bacterium Mobiculus mulieris: an approach to understand some problems for their isolation

Se hace una descripción ultraestructural de la bacteria Mobiluncus, uno de los agentes anaerobios involucrados en la causalidad del síndrome conocido como vaginosis bacteriana. M. mulieris es un bacilo curvo de ca. 3 µm de longitud, lofotrico, cuyos flagelos son subpolares. A partir del primoaislamiento pude cocultivarse accidentalmente con un coco Gram positivo y un bacilo Gram negativo, cuyas colonias, semejantes a las de Mobiluncus, miden menos de 0.2 µm de diámetro (límite de resolución del ojo humano), por lo que se recomienda analizar las placas de cultivo bajo el microscopio de disección.

Aplicación del método de coagulación de plasma al estudio ultraestructural de especímenes biológicos / Use of the plasma coagulation method for ultraestructural analysis of biological specimens

Biological particulate specimens, including Saccharomyces cerevisiae yeast, bovine spermatozoa and human blood cells (normal erytrocites and leukemic cells) were processed for scanning and transmission electron microscopy using the coagulated plasma technique. The espcimens were suspended in frozen and thawed plasma; later, coagulation was induced by adding CaCl2. The clot was cut into small pieces and processed as tissue fragments. The technique is an useful tool when processing biological particulate specimens for electron microscopy